Optimization of two-dimensional gel electrophoresis system of rice endosperm
In order to compare the difference of endosperm protein expression in grain filling period of hybrid progenies differed in amylase contents derived from the same hybrid compose, One serial of two-dimensional gel electrophoresis which was suitable to endosperm proteins of rice seed in grain filling period was set up. It was discussed on the key technologies of two-dimensional gel electrophoresis, such as the extraction, the sample loaded quantity in IEF, the transference of IPG strips and so on. The results showed that TCA-Acetone extraction was better than phenol method when the protein sample was dissolved only once, and can get the most clear and well separated two-dimensional gel electrophoresis diagram with the most protein spots, when the sample loaded quantity was 300 μg.
Kay words: riceendosperm proteintwo-dimensional gel electrophoresissystem optimization
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万方数据
维普
