Establishment and optimization of AFLP reaction system for Lilium lancifolium Thunb
An AFLP reaction system was established with Lilium lancifolium Thunb.by optimizing some factors including enzyme digestion,ligation,pre-amplification and selective amplification.The results showed that the genomic DNA extracted by the improved CTAB method was fit for AFLP analysis.The digestion duration should be 8 h at 37 ℃.The ligation should be overnight at 16 ℃.The products of pre-amplification should be diluted by 30 times.The clear and stable amplification patterns could be obtained from 12 pairs of primer combinations with the optimized system.This paper might provide a method involving in gene mapping and marker-assisted breeding of Lilium lancifolium Thunb.
NETL
NSTL
万方数据
维普
