GS/GOGAT cycle is the main assimilation ways in sugar beet. This study is based on nitrogen induction of sugar beet seedings. We processed sugar beet seedings with ranges of azaserine concentrations at different time, and quantified the GS1, GS2 and GOGAT mRNA expression level using qRT-PCR method. Simultaneously GOGAT and GS enzyme activity was also determined, further to elaborate the synegy changes of two enzymes. The results showed that after azaserine treatment of sugar beet leaves, GOGAT activity began to be inhibited at 6 h, GS activity began to be inhibited at 9 h. In addition, GOGAT mRNA expression level started to decrease at 6 h, GS1 mRNA expression level began to drop at 2 h, and GS2 mRNA expression levels started to decrease at 9 h, indicated GS2/GOGAT cycle was the main synergy way in sugar beet seedlings.
NETL
NSTL
维普
万方数据
