Effects of grafted resistant/susceptible rootstock against gray mold on tomato leaf endophytic bacterial communities
In this study,resistant/susceptible rootstocks against tomato gray mold were screened and grafted.The endophytic bacterial communities in tomato leaves were analyzed after inoculation with Botrytis cinerea by high-throughput sequencing analysis,and then explored differences in the structure and composition of the endophytic bacterial communities.Furthermore,the potential relation-ship between the differential microbial community and disease occurrence was analyzed.The results showed that grafted tomatoes with disease-resistant rootstocks significantly increased the alpha diversity of the endophytic bacteria in the leaves and the relative abundances of potentially beneficial genus such as Ochrobactrum,Bacillus,and Streptomyces compared to grafted tomatoes with susceptible rootstocks under the absence of Botrytis cinerea.After inoculation with Botrytis cinerea,grafted tomatoes with disease-resistant rootstocks showed a significant increase in the relative abundances of potentially beneficial bacterial genus Ochrobactrum and Sphingobacterium in the leaves compared to grafted tomatoes with susceptible rootstocks.Additionally,the relative abundances of OTU41 and OTU57,belonging to bacterial genus Ochrobactrum and Sphingobacterium,respectively were significantly negatively correlated with the incidence and disease index of gray mold.There were significant differences in the structure of the endophytic bacterial communities in the leaves between tomatoes grafted with resistant and susceptible rootstocks.The improvement of disease resistance in tomato grafted with disease-resistant rootstocks might be related to the enrichment of specific endophytic beneficial bacteria in the leaves.
different resistance rootstocksgraftedtomatoleaf endophytic bacteriagray moldbacterial community structure
NETL
NSTL
万方数据
