The upper respiratory tract disease of sheep is a common disease in veterinary clinical practice,and its etiology is more common due to the infection of Mannheimia haemolytica.This bacterium has multiple serotypes and is regionally prevalent,soit can cause serious economic losses in the local sheep breeding industry.This experiment collected dead sheep lung tissues with a history of respiratory diseases from the farm in Yulin,Shaanxi to explore the causes and hazards of the disease,and enrich the prevention and control measures of the disease.Morphological,biochemical,molecular biological identification,drug sensitivity testing,and mouse pathogenicity testing were performed on the isolated strains.The results showed that the isolated strain was the Mannheimia haemolytica strain SYS-1,serotype A1,with a virulence factor of LKT,which carried a lethal risk.This strain could ferment mannitol and sorbitol,but could not ferment mannose.The indole test result was negative,and the hydrogen peroxide test was positive.This strain showed slow growth,and was sensitive to drugs such as enrofloxacin and fluphenicol,and was resistant to gentamicin and erythromycin.