羊源溶血性曼氏杆菌SYS-1株分离鉴定及其毒力与耐药性分析
Isolation,identification,virulenceand drug resistance analysis of SYS-1 strain of sheep-derived Mannheimia haemolytica
刘芳 1苗苗 1乌云那日苏 2乌志勇 1白伟琴 1王金玲 1格日勒图1
作者信息
- 1. 内蒙古农业大学兽医学院,呼和浩特 010018
- 2. 锡林郭勒盟农牧局综合保障中心,内蒙古 锡林浩特 026000
- 折叠
摘要
绵羊上呼吸道疾病是兽医临床常见病,常见病因为溶血性曼氏杆菌感染.该菌具有多种血清型且呈区域性流行,造成当地绵羊养殖业严重经济损失.为探究陕西榆林羊场呼吸道疾病发病原因和危害,丰富该病防治措施,试验采集羊场有呼吸道病史的病死羊肺组织进行平板划线,对其分离的菌株进行形态学观察、生化试验及分子生物学鉴定及药敏性和小鼠致病性试验.结果表明,该菌株为A1型溶血性曼氏杆菌SYS-1株,含有毒力因子抗白细胞毒素,具有致死性风险.分离菌可发酵甘露醇和山梨醇,无法发酵甘露糖.吲哚试验结果阴性,过氧化氢试验呈阳性.该菌株生长缓慢,可对恩诺沙星、氟苯尼考等药物敏感,对庆大霉素和红霉素耐药.
Abstract
The upper respiratory tract disease of sheep is a common disease in veterinary clinical practice,and its etiology is more common due to the infection of Mannheimia haemolytica.This bacterium has multiple serotypes and is regionally prevalent,soit can cause serious economic losses in the local sheep breeding industry.This experiment collected dead sheep lung tissues with a history of respiratory diseases from the farm in Yulin,Shaanxi to explore the causes and hazards of the disease,and enrich the prevention and control measures of the disease.Morphological,biochemical,molecular biological identification,drug sensitivity testing,and mouse pathogenicity testing were performed on the isolated strains.The results showed that the isolated strain was the Mannheimia haemolytica strain SYS-1,serotype A1,with a virulence factor of LKT,which carried a lethal risk.This strain could ferment mannitol and sorbitol,but could not ferment mannose.The indole test result was negative,and the hydrogen peroxide test was positive.This strain showed slow growth,and was sensitive to drugs such as enrofloxacin and fluphenicol,and was resistant to gentamicin and erythromycin.
关键词
绵羊/溶血性曼氏杆菌/遗传进化树/毒力因子Key words
sheep/Mannheimia haemolytica/genetic evolution tree/virulence factors引用本文复制引用
基金项目
内蒙古自治区自然科学基金(2021MS03047)
出版年
2024
NETL
NSTL
万方数据