Study on yeast surface display system of Kluyveromyces lactis
Yeast surface display technology,which anchors target proteins on the cell surface,has been widely applied in the fields of vaccine preparation,drug screening,environmental management,and strain selection. In pursuit of high-efficiency production of therapeutic proteins and performing protein engineering in K. lactis,the study designed and implemented a surface display system utilizing Sed1 from Saccharomyces cerevisiae as an anchor and His Tag as a detection target. The high-throughput characterization technology that combined indirect immunofluorescence labeling and whole-cell flow cytometry was employed to comprehensively evaluate the performance of the system. Laser scanning confocal microscopy imaging further confirmed the successful display of Human serum albumin as a model therapeutic protein. Following optimization of detection conditions,a remarkable maximum display efficiency of 89.8％ was achieved. This study was the first time to establish and systematically optimize a yeast surface display system in K. lactis and laid the foundation for conducting protein engineering in K. lactis.
Kluyveromyces lactisyeast surface displayoptimizationHuman serum albumin
万方数据
维普
