Screening of corn straw degrading bacteria in the digestive tract of Protaetia brevitarsis larvae based on high-throughput microbial separation technology
Screening of corn straw degrading bacteria in the digestive tract of Protaetia brevitarsis larvae based on high-throughput microbial separation technology
Intestinal microorganisms play a key role in the transformation of corn straw by Protaetia brevitarsis larvae. In order to obtain excellent straw-degrading strains,this study optimized the extraction parameters of straw extract (SE),and prepared SE medium. A total of 1128 strains of bacteria were isolated from the hindgut of Protaetia brevitarsis larvae by using high-throughput isolation and culture. The culturable rate of microorganisms was 42.1％. Among them,409 strains of cellulose degrading bacteria and 669 strains of lignin degrading bacteria were screened. The results showed that the degradation rates of strain P61-C4,P63-G12-1 and P62-D4-2 on filter paper strips were 45.6％,31.7％ and 26.8％,respectively. The degradation rates of sodium lignosulfonate by P61-C7-1,P61-C6-2 and P61-C10-1 were 50.5％,30.6％and 25.1％,respectively. The results clarified the contribution of intestinal microorganisms to the conversion of corn straw by the larvae of Protaetia brevitarsis,which provided abundant strain resources for the biodegradation of straw. It was of great significance for promoting the sustainable development of agriculture.
关键词
白星花金龟幼虫/高通量分离培养/秸秆降解菌/16S/rRNA鉴定
Key words
Protaetia brevitarsis larva/high-throughput isolation and culture/straw degrading bacteria/identification of 16S rRNA
维普
万方数据