Purification and characterization of Panax ginseng C. A. Mey pectin SB
A water-soluble polysaccharide was isolated from the root of Panax ginseng C. A. Mey. The crude polysaccharide was fractionated and purified by deamylumized, freezed - melted, gel filtration on Sepharose CL - 4B and deproteinized, so pectin SB was abstained. The analysis of gel filtration on DEAE -Sephadex A - 25 indicated that SB was homogenous. The GC analysis indicated that SB contained the monosaccharides of GalA, Gal , Ara and Rha and their molar ratio is 1. 32:1. 00:0. 34:0.15. The gradient partial hydrolysis with acid was used for the structure analysis of SB. The results revealed that the main chain of SB is composed of GalA and Gal, the inner is GalA. The border of the main chain and the side chain and the end is composed of Gal, Ara and Rha. All results indicated that SB is an acidic het-eropolysaccharide.
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