Through boiling with hot water and precipitating with ethanol,Agaricus blazei Murrill polysaccharides were extracted from the edible fungus Agaricus blazei Murrill.By ion exchange-gel column chromatography,two homogeneous Agaricus blazei Murrill polysaccharide fractions WABM-N-a and WABM-A-b with high yield,uniform charge and molecular weight distribution were separated and purified.Structural analysis of the two homogeneous fractions shows that the WABM-N-a fraction forms the main chain skeleton structure with 1 ,6-Galp,and there are more 1 ,6-Glcp,1 ,3-Fucp structures and a small number of 1 ,4-Glcp,1 ,3-Glcp structures connected to the O-3 and/or O-4 positions of Gal,and the Glc on the side chain has a small number of branches on the O-3 position.The WABM-A-b grade is divided into 1,6-Glcp-linked dextran structures,which may all branch at O-2,O-3 and/or O-4 positions,and the side chain structure may be 1 ,3-Glcp,1 ,4-Glcp,T-Glcp,and 1 ,6-Galp.
关键词
食用菌/姬松茸多糖/分离纯化/结构分析
Key words
edible fungi/Agaricus blazei Murrill polysaccharide/separation and purification/structural analysis
维普
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