Isolation,purification and structural analysis of Agaricus blazei Murrill polysaccharides from edible fungi
Through boiling with hot water and precipitating with ethanol,Agaricus blazei Murrill polysaccharides were extracted from the edible fungus Agaricus blazei Murrill.By ion exchange-gel column chromatography,two homogeneous Agaricus blazei Murrill polysaccharide fractions WABM-N-a and WABM-A-b with high yield,uniform charge and molecular weight distribution were separated and purified.Structural analysis of the two homogeneous fractions shows that the WABM-N-a fraction forms the main chain skeleton structure with 1 ,6-Galp,and there are more 1 ,6-Glcp,1 ,3-Fucp structures and a small number of 1 ,4-Glcp,1 ,3-Glcp structures connected to the O-3 and/or O-4 positions of Gal,and the Glc on the side chain has a small number of branches on the O-3 position.The WABM-A-b grade is divided into 1,6-Glcp-linked dextran structures,which may all branch at O-2,O-3 and/or O-4 positions,and the side chain structure may be 1 ,3-Glcp,1 ,4-Glcp,T-Glcp,and 1 ,6-Galp.
edible fungiAgaricus blazei Murrill polysaccharideseparation and purificationstructural analysis
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