The effect of imidacloprid stress on the antioxidant and detoxification enzyme systems of Bombus ignitus Smith
To explore the sublethal dose of imidacloprid stress on the effect of Bombusignitus workers, detoxification enzyme activity and detoxification gene expression.The detoxification genes were cloned by the RACE (rapid amplification of cDNA ends )technique,the expression levels and antioxidant enzyme activity of them in the 2l day-old of Bombus ignitus after treatment were detected,melatonin was also fed to improve the resistance of bumblebees.The results showed that,With the increasing duration of the stress period,The significant decrease in survival in the imidacloprid processing group (IMI group),But always lower than imidacloprid + melatonin processing group (IMI + MT group);Expression of the eight detoxification genes in the IMI group,in addition to AChE,DDTase and GLD, the rest of the expression levels increased with the extension of imidacloprid stress,and significantly higher than CK group and lower than IMI + MT group;The expression of DDTase in the IMI group was not significantly different from that in the CK group,but were consistently lower than those in the IMI + MT group,suggesting that the effect of imidacloprid on DDTase is insignificant.However, melatonin induced DDTase expression significantly (P<0.05);The expression level of both AChE and GLD in the IMI group was lower than that in the CK group,detrend with the extension of imidacloprid stress.However,the expression level in the IMI + MT group was always higher than that in the IMI group.In conclusion,short-term sublethal dose of imidacloprid stress has no significant effect on Bombus ignitus,while long-term stress affects their survival rate,and the metabolic detoxification of pyridacloprid is a physical and chemical process involving multiple enzyme lines and multiple genes,and melatonin can improve the resistance of Bombusignitus.
万方数据
维普
