Study on the microbial community structure of Pleurotus citrinopileatus cultivated with chicken manure and deer manure fermentation materials during the cultivation process
In order to explore the effects of adding deer manure and chicken manure on the microbial diversity and community structure of Pleurotus citrinopileatus during the cultivation of Pleurotus citrinopileatus with Pleurotus citrinopileatus fermented feed,the bacterial and fungal communities in the pre-wetting stage,fermentation stage,mycelium colonization stage and fruiting body formation stage of Pleurotus citrinopileatus were analyzed by MiSeq high-throughput sequencing technology. The results showed that 24483 and 1324 effective OTU were produced by bacteria and fungi,respectively,at the 97％ similarity level of the genetic material in the variable region of the 12 composts.There was no significant difference in alpha diversity between bacterial and fungal communities under different manure treatments.Bacterial genera such as Fibrobacter,Sphingomonas,and fungal genera such as Trichosporon and Thermophilic Fusarium with straw degradation function were identified.There were significantly different markers in the bacterial community in the culture of deer manure and chicken manure treatment,but no significantly different markers were found in the mixed group of chicken manure and deer manure.The results showed that the substrate fungi were mainly involved in the degradation of the substrate,and the bacteria of the substrate played an important role in the process of straw degradation,mycelial growth and fruiting body formation.The species and abundance of bacteria and fungi in chicken manure fermentation material and chicken manure and deer manure mixed fermentation material had stronger effects on straw degradation and mycelial growth than deer manure fermentation material.The results revealed the changes of microbial diversity and community structure during the cultivation of Pleurotus citrinopileatus fermented material,which provided a theoretical basis for analyzing the cultivation mechanism of Pleurotus citrinopileatus fermented material.
Pleurotus citrinopileatushigh-throughput sequencingfermentation material cultivationmicrobial diversity
万方数据
维普
