Study on the mechanism of Weibimei promote bismuth clearance through autophagy
The drug of Weibimei is a kind of antimony drug for the clinical treatment of Helicobacter pylori.The purpose of this study aimed to explore the effect of Weibimei on the obviation heavy metal bismuth in cells and its associated mechanism.Atomic fluorescence absorption method,western blotting,real-time quantitative PCR,MDC(cadaver dandanamide)staining and other experiments were used to explore the effect of gastric bismuth magnesium on the autophagy function of cells.The results showed that the residual amount of bismuth in the gastric bismuth magnesium group was 20％of that in the bismuth aluminate group.The protein expression of autophagy-related proteins LC3B-Ⅱ,Beclin1,ATG7,ATG5 and ATG16L in the gastric bismuth magnesium group was increased followed with the increased and the expression of p62 protein decreased,and the transcription levels of Beclin1 and various ATG genes.At the same time,the number of autophagosomes in gastric bismuth magnesium cells increased significantly.So Weibimei Drug achieved the scavenging effect on intracellular heavy metal bismuth by increasing the cellular autophagy.
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