首页|NudCL2 is required for cytokinesis by stabilizing RCC2 with Hsp90 at the midbody

NudCL2 is required for cytokinesis by stabilizing RCC2 with Hsp90 at the midbody

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Cytokinesis is required for faithful division of cytoplasmic components and duplicated nuclei into two daughter cells.Midbody,a protein-dense organelle that forms at the intercellular bridge,is indispensable for success-ful cytokinesis.However,the regulatory mechanism of cytokinesis at the midbody still remains elusive.Here,we unveil a critical role for NudC-like protein 2(NudCL2),a co-chaperone of heat shock protein 90(Hsp90),in cytokinesis regulation by stabilizing regulator of chromosome condensation 2(RCC2)at the midbody in mam-malian cells.NudCL2 localizes at the midbody,and its downregulation results in cytokinesis failure,multinu-cleation,and midbody disorganization.Using iTRAQ-based quantitative proteomic analysis,we find that RCC2 levels are decreased in NudCL2 knockout(KO)cells.Moreover,Hsp90 forms a complex with NudCL2 to stabilize RCC2,which is essential for cytokinesis.RCC2 depletion mirrors phenotypes observed in NudCL2-downregulated cells.Importantly,ectopic expression of RCC2 rescues the cytokinesis defects induced by NudCL2 deletion,but not vice versa.Together,our data reveal the significance of the NudCL2/Hsp90/RCC2 pathway in cytokinesis at the midbody.

cytokinesismidbodyNudCL2Hsp90RCC2

Xiaoyang Xu、Yuliang Huang、Feng Yang、Xiaoxia Sun、Rijin Lin、Jiaxing Feng、Mingyang Yang、Jiaqi Shao、Xiaoqi Liu、Tianhua Zhou、Shanshan Xie、Yuehong Yang

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Department of Cell Biology,Institute of Gastroenterology of the Second Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310009,China

Research Center for Children's Health and Innovation,Binjiang Institute of Zhejiang University,Hangzhou 310053,China

Department of Biochemistry,Purdue University,West Lafayette,IN 47907,United States

Cancer Center,Zhejiang University,Hangzhou 310058,China

Center for RNA Medicine,International Institutes of Medicine,the Fourth Affiliated Hospital of Zhejiang University School of Medicine,Yiwu 322000,China

Children's Hospital,Zhejiang University School of Medicine,National Clinical Research Center for Child Health,Hangzhou 310052,China

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2024

10.1093/procel/pwae025

蛋白质与细胞

蛋白质与细胞

CSTPCD
影响因子：0.645
ISSN：
年,卷(期)：2024.15(10)