首页|防治大豆胞囊线虫芽孢杆菌初步筛选

防治大豆胞囊线虫芽孢杆菌初步筛选

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大豆胞囊线虫(Heterodera glyclnes Ichlnohe)是大豆根部的重要寄生线虫,严重威胁大豆生产,而且防治困难.目前人们试图利用生物防治的方法来控制大豆胞囊线虫病害,以达到农业可持续发展的目的.芽孢杆菌是一类重要的微生物资源,该类微生物具有良好的抗逆性,在线虫病害的生物防治方面具有一定的发展前途.采用稀释分离法,从东北和华北等地区采集土样分离获得295株芽孢杆菌,通过筛选获得6株对大豆胞囊线虫有明显抑制作用的菌株,分别是B12、1328、1349、1365、1376和B108.其中B12菌株的菌悬液对大豆胞囊线虫有较强的杀死作用,校正死亡率达到74.03%;I]65发酵液,处理48 h后校正死亡率达到85.90%;B12、B65、1349对大豆胞囊线虫抑制率分别达89.50%、89.50%、88.00%,均与对照差异显著.这些菌株对大豆胞囊线虫的形成表现出明显的抑制作用,有可能成为非常有应用前景的生防菌株.
Screening of Bacillus sp.Against Soybean Cyst Nematode(Heterodera glycines)
The Soybean Cyst Nematode(Heterodera glycines) ,causes economically significant damage to soybean in China. Some bacteria Bacillus,axe both resistant to advese envimment and against pathogens. 295 Bacillus sp. were isolated from soil samples by diluted separation in Northeast and North China. B12, B28, B49, B65, B76 and B108 strains could suppress Heterodera glycines and strain suspentions of B12 was effective significantly. The corrected mortality rate of nematodes of strain suspentions with B12 were 74.03% after 48 h. The corrected mortality rate of nematodes of fermentation filtrate with B65 were 85.90% after 48 h. The suppressive percentages of cysts hatching of B12, B65 and B49 were 89.50,89.50 and 88.00. The bacterial strains which had effect on the nematode would be used as the bio- control agents on the soybean root rot pathogen and soybean cyst nematode.

Heterodera glycinesBacillus sp.Fermentation brothCorrect mortality rate

段玉玺、于海峰、陈立杰、王胜君

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沈阳农业大学植物保护学院,北方线虫研究所,辽宁,沈阳,110161

大豆胞囊线虫 芽孢杆菌 发酵液 校正死亡率

国家自然科学基金科技部农业科技成果转化基金

3067319905EFN212100059

2008

大豆科学
黑龙江省农业科学院

大豆科学

CSTPCDCSCD北大核心
影响因子：0.641
ISSN：1000-9841
年,卷(期)：2008.27(5)
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