The line JLCMS82A and its maintainer line JLCMS82B were used in this trial with a split block design for evaluating the effects of cross-pollination of alfalfa leafcutting bees in different treatments of bee amount and row cytoplasmic male sterile ( CMS) ratio of male and female parents. The results showed that the seed setting of the CMS line and the whole yield of the male and female parents existed significant difference at the 1% level and the yield of the CMS line at the 5% level among the different treatments of bee amount. There was significant difference at the 1% level in the seed setting of the CMS Line and the whole yield of the male and female parents, whereas the difference of the yield of the CMS line was not significant among the different treatments of row ratio of male and female parents. There was no significant difference between the treatments of bee amount and row ratio of male and female parents. In the treatment of different bee amount, the highest seed setting and yield of the CMS line and the whole yield of the male and female parents were observed when the amount of alfalfa leafcutting bees reached 30,000/ha. In the treatment of different row ratio of male and female parents, the highest seed setting and whole yield of the male and female parents were also observed when the row ratio was 1: 1, whereas there was no significant difference for the yield of the CMS line between the different row ratio. On the whole, the best effective treatment was that of the 1:1 row ratio with the amount of 30,000 alfalfa leafcutting bees per hectare.