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大豆耐旱选择群体基因型分析与株高QTL定位

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以红丰11为轮回亲本、Clark为供体亲本构建回交群体进行耐旱性鉴定,并对获得的选择群体进行全基因组SSR标记扫描.计算供体基因型导人频率,同时利用卡方测验检测偏分离SSR位点,对株高进行QTL定位.结果表明:卡方测验检测到11个SSR偏分离位点(超导入)分布于8条连锁群;株高共定位8个QTL位点分布于A<,1>、C<,2>、E、F、L、M和O连锁群.在C<,2>连锁群的Satt305位点,耐旱供体等位基因显著性检测和株高QTL位点具有一致性;在E连锁群的Sat_136位点,F连锁群的Satt586和GMRUBP位点,L连锁群的Satt156位点,M连锁群的GMSL514位点均与有一致性,这些位点可能是控制大豆耐旱性的重要位点.
QTL Identification of Plant Height and Analysis of Genotype to Soybean in Selection Population
A primary backcross introgression soybean population was constructed by using Hongfeng 11 as recurrent parent and Clark as donor parent. After screened by drought stress, the genotype of selective population were obtained with the whole genome SSR markers, and the frequency of donor genes segments had been analyzed. QTLs of plant height was mapped by Chitest analysis. In total, 11 SSR excessive introgression on 8 chromosomes were detected with X2 test. The QTL identification was conducted by one-way ANOVA (for single marker analysis, P <0.01 ). Eight QTLs of RPH were found in A1 , C2, E,F, L, Mand O linkage groups. The QTL at Satt305 in C2 linkage group was coincident with excessive introgression of X2 test.The same with Sat_136 in E linkage group, Satt586 and GMRUBP in F linkage group, Satt156 in L linkage group, GMSL514 in M linkage group. So these QTL were the essential loci for drought tolerance, and the foundation in fine mapping, cloning and molecular breeding of favorable genes related with drought tolerance.

SoybeanDrought tolerancePlant heightQTL identification

李灿东、蒋洪蔚、郭泰、王志新、吴秀红、郑伟、陈庆山、胡国华

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黑龙江省农垦科研育种中心,黑龙江,哈尔滨,150090

黑龙江省农业科学院佳木斯分院,黑龙江,佳木斯,154007

东北农业大学,农学院,黑龙江,哈尔滨,150030

国家大豆工程技术研究中心,黑龙江,哈尔滨,150050

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大豆 耐旱性 株高 QTL分析

农业重大专项资助项目农业公益性行业科研专项资助项目

2009ZX08009-13B200903003

2011

大豆科学
黑龙江省农业科学院

大豆科学

CSTPCDCSCD北大核心
影响因子:0.641
ISSN:1000-9841
年,卷(期):2011.30(1)
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