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大豆花叶病毒诱导的野生大豆抑制差减文库构建及初步分析

Construction and Primary Analysis of a SSH Library of Wild Soybean Induced by SMV

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 维普
  • 万方数据
以野生大豆Bian0526为试验材料,通过抑制性差减杂交技术(SSH)构建了大豆花叶病毒(SMV)诱导的cDNA 差减文库.在文库中共筛选到15343个阳性克隆,PCR鉴定插入片段大部分集中在500～800 bp之间.利用BLAST 在GenBank文库中进行序列相似性比对,获得有功能的EST 200个.对有功能注释的同源分析发现,其功能涉及植物的细胞自身保护和系统获得性抗性等方面,说明这些基因很可能参与到野生大豆抗大豆花叶病毒反应中.
To isolate the genes related to wild soybean resistance in Soybean Mosaic Virus(SMV) .Suppression Subtractive Hybridization (SSH) was utilized to construct the different expression cDNA library using wild soybean variety Bian0526 inoculated with race 3 of SMV. From this library,a total of 15 343 positive clones were screened,and the length of most of the insert fragments was focused on 500-800 bp. ESTs similarity analysis of these unigenes was performed with the Blastx and Blastn a-gainst the non-redundance database in GenBank. The results showed that 200 functional ESTs had been gotten, and they were related to cell self-protection and system acquired resistance, etc. This indicated that these genes might be involved in resistance to SMV of wild soybean.

Wild soybeanSoybean Mosaic VirusSuppression subtractive hybridization (SSH)cDNA library

史凤玉、朱英波、龙茹、武云鹏、乔亚科

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河北科技师范学院生命科技学院,河北昌黎066600

野生大豆 大豆花叶病毒病 抑制差减杂交技术(SSH) cDNA文库

国家自然科学基金河北省自然科学基金河北省自然科学基金河北省科技厅资助项目河北省科技厅资助项目河北省教育厅资助项目

30940050C2006000559C200900086806547005D-309220103D-11Z2008114

2011

大豆科学
黑龙江省农业科学院

大豆科学

CSTPCDCSCD北大核心
影响因子：0.641
ISSN：1000-9841
年,卷(期)：2011.30(6)
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