Observation of Soybean Ultrastructure with Different Phosphorous Treatments
Eight soybean cultivare with different P efficiency were cultivated until flowering in 3 P levels (0, 640 and 960 mg ?kg-1' ) and then took the mid - leaf of the second ternate for ultrastructure observation. The result showed that with the increase of P amount, the stoma number, size and aperture in unit leaf area of high or low P efficiency cultivars were increased. Under PO control, the stoma size of high P efficiency cultivar was smaller than low P ones, but the aperture was bigger than low P cultivars. The chloroplast number in single mesophyll cell of high P efficiency cultivars in low and middle P levels were significantly higher than low P efficiency ones, but opposite in high P treatment. With the increase of P amount, the chloroplast number was increased with regular shape. However, a large amount of P fertilizer may be harmful to the chloroplast of high P efficiency genotypes and destroyed chloroplast shape seriously. In conclusion, scientifically P utilization could improve the ultra microscopic structure of stomata and chloroplast, finally increase the efficiency of photosynthesis.