A study was conducted in lab and greenhouse to determine the effect of the urea on eggs, second-stage juveniles ( J2 ) of Heterodera glycines and control efficiency of Soybean Cyst Nematode ( SCN) . The results showed that the urea inhibited the nematode egg hatch and the activity of J2 at the concentration of urea from 0.03 to 1.0 mol·L-1 ,and with the increase of concentration,the suppressive effect rose accordingly. The suppressive rate of egg hatching and mortality of J2 reached 96% and 100% when urea concentration was at 1.0 mol·L-1,respectively. In greenhouse,application of urea significantly reduced the population of SCN in soil and soybean root. The application of urea also enhanced height,fresh and dry weight of shoots and roots of soybean,and the increase rate of fresh weight was 42. 8%. The urea has lethal effect on SCN,and the population of SCN is suppressed by urea in a certain extent.