Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13"
Mutants and mutant population are the foundation for gene function analysis and crop genetic improvement. Seed of soybean cultivar "Jihuang 13" were treated with ethyl methane sulfonate(EMS) , sodium azide(NaN3) and nitrogen ion beam implantation respectively for screening mutants and constructingmutant population. Fifteen stem related mutants, 19 leaf related mutants,31 floral related mutants,3 seed related mutants,and 18 maturity related mutants were obtained after selected in M2 generation,and validated in M3 and M4 generation. Because there were 9 mutants possessing composite phenotypes,77 genetically stable mutants were finally screened out. Further genetic analyses for a male sterile mutant and a black seed color mutant were made in F2 segregating population respectively. The results showed that the phenotypes of these two mutants were both controlled by a single recessive gene. The mutants and mutant population obtained would lay a foundation for functional genom-ics analyses and genetic improvement of soybean.
国家科技期刊平台
NSTL
万方数据
维普
