Bioinformatics Analysis of NAC Gene Family in Glycine max L.
Genes containing the NAC domain (NAC family genes)are plant-specific transcriptional regulators and expressed in various developmental stages and tissues.We performed a bioinformatics analysis of NAC family genes in soybean.Based on bioinformatics methods,taking advantage of soybean database combined with public database(NCBI),we identified 152 NAC proteins from soybean genome.NAC domains from both predicted and known NAC family proteins were classified into ten subgroups by sequence similarity.Further genetic mapping of NAC genome localization found that they distributed on 20 chromosomes which the twelfth chromosome exist the most.We also predicted and analyzed their amino acid composition,physical and chemical characteristics,as well as secondary structures.The research found that the number of amino acid and hydrophobic of amino acid sequences in different subfamilies presented some differences.Meanwhile,the results of secondary structure predicted that the main composition of 152 predicted NAC proteins among them were random coil and alpha helix.The results of this investigation could definitely provide a significant foundation for further research on the function analysis of soybean NAC gene family.