Internal Transcribed Spacer (ITS) sequence identification of rDNA in a Pythium strain PSHH1
Pythium is an important part of soybean root rot fungal population in Heilongjiang province,in order to determine the species of Pythium in Heilongjiang province,PSHH1 has been isolated from the soil of soybean root in Heihe city.And rDNA-ITS sequence analysis was applied to PSHH1.According to the results of the analysis of rDNA-ITS sequences,the sequence homology was more than 99％ when it was compared with the sequence of registration number AB259316.1 and AB512973.1 in GeneBank.Therefore,we think PSHH1 was a Pythium sylvaticum.In order to study the pathogenicity of PSHH1,using seed rot method and hypocotyl inoculation method,60 main soybean cultivars were selected to determine the pathogenicity of PSHH1,and seed rot rate and stem discount rate can be reflected to evaluate the pathogenicity of PSHH1,results showed that PSHH1 had strong pathogenicity to 13.3％ main soybean cultivars,at the same time,there were 61.67％ main soybean varieties expressed weak pathogenicity to PSHH1.
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