豆豉中5株产纤溶酶菌的筛选与鉴定
Isolation and Identification of Five Fibrinolytic Enzyme Producing Strains from Douchi (Chinese Traditional Food)
吕美云 1刘紫英1
作者信息
- 1. 宜春学院化学与生物工程学院,江西宜春336000
- 折叠
摘要
采用酪蛋白平板初筛的方法,从全国14种纳豆和豆豉中分离到39株具有蛋白酶活力的菌株;又以猪血粉平板进行复筛,并采用纤维蛋白平板法测定纤溶酶活力,得到5株产纤溶酶能力较强菌株,分别命名为CQDC-03、CWND-03、NCDC-02、YJND-01和HDC-02.对其进行16S rDNA序列分析,结果表明:CWND-03、CQDC-03和Bacillus subtilis相似性为99％,NCDC-02和Bacillus subtilis相似性为98％.因而CQDC-02、CWND-03和NCDC-02认定为Bacillus subtilis,YJND-01和FJDC-02是否为Bacillus subtilis的新种还有待进一步鉴定,但确定其为Bacillus属.
Abstract
Thirty-nine strains of protease-producing strains were isolated from 14 kinds of traditional food natto and Douchi by using casein-plate method and 5 strains of highest fibrinolytic enzyme producing strains were screened by both pig blood powder-plate and fibrin plate method.The 5 strains were named NCDC-2,CQDC-03,CWND-03,YJND-01 and FJDC-02,respectively.16S rDNA sequence analysis showed NCDC-02 was similary with Bacillus subtilis by 98％,while CQDC-03 and CWND-03 were by 99％.Thus NCDC-2,CQDC-03,CWND-03 were identified as Bacillus subtilis.Further research is needed to identify whether YJND-01 and FJDC-02 are the new species of Bacillus subtilis.But it is no doubt that it is kind of Bacillus spp.
关键词
纤溶酶/筛选/16S/rDNA/鉴定/枯草芽孢杆菌Key words
Fibrinolytic enzyme/Screened/16S rDNA/Identification/Bacillus subtilis引用本文复制引用
基金项目
江西省宜春市社会发展类科技计划(JXYC2011KSA002)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据