首页|大豆品种郑97196对疫霉根腐病的抗性遗传分析及基因定位

大豆品种郑97196对疫霉根腐病的抗性遗传分析及基因定位

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  • 国家科技期刊平台
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由大豆疫霉引起的大豆疫霉根腐病是一种世界性大豆病害,对产量品质影响极大.利用抗病品种进行防治是目前最经济、有效的方法.大豆对疫霉菌株的抗性分为由单基因控制的完全抗性和由多基因控制的部分抗性两种.以对多个大豆疫霉生理小种具有抗性的大豆品种郑97196作为抗性亲本与大豆感病品系X242003杂交构建F2∶3重组自交家系群体,用大豆疫霉菌株HeN35对亲本及F2∶3群体进行抗性遗传分析并利用SSR技术对郑97196的抗性基因进行定位.结果表明:郑97196对大豆疫霉抗性是由一对显性单基因控制的,抗病对感病表现为显性,再用9种毒力公式不同的疫霉菌株分别接种郑97196和14个国内外公认的含有单个抗病基因的大豆品种(鉴别寄主),观察并记录抗性反应类型,结果显示郑97196的抗性反应类型与14个鉴别寄主有所不同,推测其可能含有新的抗病基因,暂命名为RpsZheng.通过SSR分子作图分析,该基因位于大豆分子遗传图谱N连锁群上satt485和satt584之间,与这两个标记之间的距离分别为2.1和3.7 cM.
Genetic Analysis of Resistance to Phytophthora sojae and Mapping of Resistance Gene in Soybean Cultivar Zheng 97196
Phytophthora root rot of soybean is a worldwide soybean diseases caused by Phytophthora sojae (P.sojae),has a great impact on the yield and quality.Resistant cultivars is the most economical and effective method to prevent it.Soybean Phytophthora resistance contain complete resistance controlled by single gene and partial resistance controlled by multiple genes,in this study the cultivars Zheng 97196 to multiple physiological strain of P.sojae resistant.A population of F2∶3 recombinant inbred lines(RIL) derived from the cross of Zheng 97196 × X242003,then the RIL was used to analyze the inheritance of resistance by inoculated P.sojae HeN35 and map the resistance gene to a linkage group.The results showed that the resistance of Zheng 97196 to P.sojae was controlled by a single gene (Rps),and the resistance was dominant to susceptible,then 9 kinds of different virulence formula P.sojae were inoculated Zheng 97196 and 14 widely recognized as disease-resistant soybean varieties contain a single gene(differential hosts),recorded the type of reaction,the results showed Zheng 97196 reaction type was different from 14 differential hosts,speculated that it may have new resistance genes,temporarily named RpsZheng.Basis of linkage analysis with SSR markers the gene was located on molecular linkage group N and flanked by satt485 and satt584 with genetic distances 2.1 and 3.7 cM.

Glycine maxPhytophthora root rot of soybeanGenetic analysisResistance geneSSR markers

张海鹏、郭娜、牛景萍、黄婧、彭洋、王海棠、赵晋铭、邢邯

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南京农业大学大豆研究所/国家大豆改良中心/农业部大豆生物学与遗传育种重点实验室(综合)/作物遗传与种质创新国家重点实验室,江苏南京210095

大豆 大豆疫霉根腐病 遗传分析 抗病基因 SSR标记

国家公益性行业(农业)科研专项经费优质高产高油大豆种质创新与新品种选育江苏省自然科学基金国家现代农业产业技术体系江苏省现代作物生产协同创新中心

201303018BE2013350BK2011637和BK20130684CARS-04-PS10

2016

10.11861/j.issn.1000-9841.2016.03.0373

大豆科学
黑龙江省农业科学院

大豆科学

CSTPCDCSCD北大核心
影响因子：0.641
ISSN：1000-9841
年,卷(期)：2016.35(3)
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