Establishment of SSR Fingerprint ID for New Soybean Varieties in Jilin
Currently a novel method should be developed for the rapid identification and accurate evaluation of new soybean varieties.In this paper,a total of 98 released soybean varieties in Jilin were studied with SSR markers.Twelve SSR markers that had clear,stable and reproducible electrophoretic maps were selected from 320 randomly selected SSR markers located on each chromosome of soybean.In order to distinguish effectively soybean varieties with minimal primer pairs and reveal rich genetic diversities between the test materials,7 core SSR markers were selected from aforementioned 12 SSR markers according to diversity index.At last,the bands amplified by each of the 7 core SSR markers were coded,and then the codes were combined as a fingerprint ID for soybean varieties.The results showed that each tested soybean variety had unique SSR fingerprint ID,and 7 core SSR markers were reliable and efficient.In addition,using the whole band-types amplified by each of the 7 core SSR markers coded as fingerprint ID for soybean varieties can reduce complexity effectively in recording polymorphic amplification profiles.This paper not only improve the establishment method of SSR fingerprint ID for new soybean varieties,but also meet the requirements of molecular identification for new soybean varieties in Jilin province and even the whole country.
国家科技期刊平台
NSTL
万方数据
维普
