SSR Marker Diversity of Newly Developed Soybean Breeding Lines from Huang-Huai-Hai Region
Genotyping analysis of molecular markers is an important way to evaluate the diversity of crop varieties,discover and effectively utilize elite germplasm.The objective of this study is to reveal the genetic basis of newly developed soybean lines/cultivars in Huang-Huai-Hai area.Total 60 microsatellite (SSR) molecular markers distributing evenly through soybean genome were used to genotype 284 lines.A total of 363 alleles were detected for the 60 SSR markers,with an average allele variation of 6.05 for each locus.The PIC index ranged from 0.297 to 0.849 with an average of 0.614.The average allele variation of soybean lines in Huang-Huai-Hai area was 5.75,and the PIC index was 0.604,showing a high diversity.In different provinces,Beijing and Hebei showed the highest diversity.Based on SSR data clustering analysis,the test materials could be divided into five clusters,the clustering result was related to the geographical origin.Eight pairs of SSR primers were selected to build the fingerprints of all 284 lines,these marker allele combinations could distinguish all those materials.
国家科技期刊平台
NSTL
万方数据
维普
