大豆响应盐胁迫NAC转录因子的鉴定
Identification of NAC Transcription Factors Response to Salt Stress in Soybean
袁嘉志 1高超升 1植健怡 1程玉汉 2程群 1董利东1
作者信息
- 1. 广州大学生命科学学院/广东省植物适应性与分子设计重点实验室,广东广州 510006
- 2. 北京中农富通园艺有限公司,北京 100083
- 折叠
摘要
盐胁迫是影响大豆生长发育和产量的重要环境因素之一.NAC转录因子家族是植物响应盐胁迫的重要基因家族,但在大豆中其功能尚未被完全解析.为了挖掘控制大豆耐盐的新基因,本研究首先利用全基因组分析和聚类分析,并结合盐胁迫下大豆植株的转录组数据,对大豆受盐胁迫诱导表达的NAC基因进行分析;其次,对在根中受盐胁迫诱导表达的NAC基因进行单倍型、耐盐指数和核酸多态性分析.结果显示:在大豆基因组中共鉴定出182个NAC基因,将其分成7个亚家族;18个NAC基因受盐胁迫诱导显著上调表达,其中有8个在根中表达水平较高,可能参与响应盐胁迫.我们鉴定到NAC23的一个优异单倍型Hap1,能够显著促进大豆对盐胁迫的耐性.同时,发现NAC23的Hap1单倍型在栽培大豆品种中受到了强烈的人工选择.以上研究结果为更加深入地解析NAC家族基因与大豆耐盐性之间的关系提供了新的见解,为培育高效抗逆大豆新品种提供基因资源和新的思路.
Abstract
Salt stress is one of the important environmental factors affecting the growth,development and yield of soybean.NAC family transcription factors are important genes in plant response to salt stress,but their functions have not been fully elucidated in soybean.To identity new genes that control salt tolerance in soybean,in this study,the whole genome analysis,cluster analysis and transcriptome data of soybean plants under salt stress were first used to identify NAC genes induced by salt stress.Secondly,the haplotype,salt tolerance index and nucleic acid polymorphism of NAC genes induced by salt stress in roots were analyzed.We identified 182 NAC genes in the soybean genome and divided them to 7 subfamilies.We found that the expression of 18 NAC genes were significantly up-regulated under salt stress,among which eight NAC genes were expressed in roots,which might be related to response to salt stress.An important gene NAC23 that may respond to salt stress was identified.Our results also showed that the Hap1 haplotype of NAC23 may promote the tolerance of cultivated soybean to salt stress,and this gene was subjected to strong artificial selection.These results provide new insights for the comprehensive analysis of the relationship between NAC gene and soybean salt tolerance,and provide gene resources and new ideas for breeding new varieties of highly resistant crops.
关键词
大豆/NAC转录因子/耐盐性/转录组/人工选择Key words
soybean/NAC transcript factors/salt tolerance/transcriptome/artificial selection引用本文复制引用
出版年
2023
国家科技期刊平台
NSTL
维普
万方数据