摘要
为筛选出大豆芽期耐旱种质资源,本研究采用20％的聚乙二醇(PEG-6000)模拟干旱胁迫对来自国内外的258份大豆资源开展芽期耐旱鉴定,以发芽势、发芽率、发芽指数、相对发芽势、相对发芽率、相对发芽指数和干旱伤害率为鉴定指标,最后通过隶属函数值对大豆资源芽期耐旱性进行评价及聚类分析.结果表明:胁迫条件下,各指标在品种间均差异显著;相关性分析表明隶属函数与各指标显著正相关,干旱伤害率与其他各指标呈显著负相关.根据隶属函数值将258份种质资源分成4类:耐旱型品种(系)9个;次耐旱型品种(系)55个;次敏感型品种(系)186个;敏感型品种(系)8个.通过隶属函数聚类分析将258份材料分成了 3类:耐性材料17份,如吉林小粒豆7号、东大2号等,占总体材料的6.59％;中间型99份,如垦丰13、垦农18等,占总体材料的38.37％;敏感材料142份,如黑河38、龙豆2号等,占总体材料的55.04％.研究结果将为大豆耐旱育种及芽期耐旱遗传机制研究提供适宜种质资源.
Abstract
In order to screen the drought-tolerant germplasm resources of soybean at germination stage,this study used 20％polyethylene glycol(PEG-6000)to simulate drought stress to identify the drought tolerance of 258 soybean resources at germination stage from home and abroad.The germination potential,germination rate,germination index,relative germination potential,relative germination rate,relative germination index and drought damage rate were used as identification indexes.Finally,the drought tolerance of soybean resources at germination stage was evaluated and clustered by membership function value.The results showed that there were significant differences among the stress treatments,and the correlation analysis showed that the membership function was positively correlated with each index,and the drought injury rate was negatively correlated with each index.According to the membership function,258 germplasm resources were divided into 4 categories:There were 9 varieties(lines)of drought-tolerant,55 varieties(lines)of sub-drought-tolerant,186 varieties(lines)of sub-sensitive and 8 varieties(lines)of sensitive.The 258 materials were classified into 3 types by the subordinate function cluster analysis:17 tolerant materials,such as Jilin xiaolidou 7,Dongda 2,etc.,which accounted for 6.59％of the total materials,99 intermediate materials,for example,Kenfeng 13 and Kennong 18 accounted for 38.37％of the total materials,and 142 sensitive materials,such as Heihe 38 and Longdou 2,accounted for 55.04％of the total materials.The results will provide suitable germplasm resources for drought-tolerant breeding and genetic mechanism of drought-tolerant in bud stage.
基金项目
吉林省农业科技创新工程人才基金—研究生基金(c12040414)
现代农业产业技术体系建设专项(CARS-04-PS11)
2019年度省科研院所引进高层次科技创新人才资助计划()
吉林省农业科技创新工程项目(CXGC2022DX002)
中青年科技创新创业卓越人才项目(20220508105RC)
国家科技期刊平台
NSTL
维普
万方数据