Flanking Sequence of Exogenous Insertion in Salt-tolerant Transgenic Soybean Event AtARA6-A001 and Its Application
AtARA6 gene from Arabidopsis thaliana was introduced into soybean receptor Shennong 9 by Agrobacterium-mediated transformation of soybean cotyledon nodes,obtaining the salt-tolerant transgenic soybean event AtARA6-A001.To identify the molecular characteristics and setting up a detection method of this transgenic soybean material,we could further accelerate the biosafety evaluation of this transgenic event in the future.In this study,the copy number of exogenous gene of the homozygous transgenic soybean AtARA6-A001 was identified by Southern hybridization as well as the position and direction of the insertion site.Meanwhile,the left and right flanking sequences of exogenous T-DNA were obtained by PCR amplification.Based on these data,a specific qualitative PCR method for detecting AtARA6 gene salt-tolerant soybean A001 event was established.This method has high specificity,and can quickly identify transgenic soybean events,including AtARA6-A001 parents,derivatives,or varieties,as well as plants,tissues,seeds and products for specific detection,achieving effective supervision and management of transgenic soybeans by technical support.
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