Genome-wide Identification and Expression Analysis of C2H2 Zinc Finger Protein Gene Family in Soybean
In order to further search for potential candidate genes related to soybean yield,44 C2 H2 zinc finger protein family members from the genome database of soybean(Glycine max)were classified into 11 subfamilies by BLAST mapping of Arabidopsis thaliana L.C2 H2 zinc finger protein sequences using bioinformatics and transcriptomics technologies.The 44 C2H2 zinc finger protein family members screened from the soybean genome database were divided into 11 subfamilies,and analysed for phylogeny,conserved structural domains,cis-acting elements,sequencing data and transcriptome data.The results showed that the C2H2 zinc finger protein family members were distributed in 17 soybean chromosomes,and the structural domains of the genes in each subfamily were similar and highly conserved.The promoters of the members contained regulatory elements for stress and hormone resistance,and the RNA-seq results showed that eight genes were differentially expressed in different plant tissues.Quantitative real-time PCR analysis showed that three genes analysed were transcriptional regulators of the SUPERMAN,GmC1-1 iZFP42 was the corresponding gene in the Ln locus,which is involved in the regulation of soybean leaf shape and seed,GmC1-1iZFP12 and GmC1-1iZFP30 were transcriptional regulators of the SUPERMAN gene,and GmC1-1iZFP21 may be involved in the process of soybean grain formation and development,and is a candidate gene for regulating related processes.This study provides directions for further searching and mining of soybean high yield genes,as well as references and insights for the use of C2H2-type zinc finger proteins and bioinfonnatics in plants.
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