Analysis of Factors Causing Soybean Crinkle Leaf in Southern China Soil
To identify the causes of soybean leaf wrinkling in southern China,this study explored and studied from two perspectives:Non biological and biological factors in the soil of southern China.Differential mixing pool method and ICP-OES analysis technology was used to study the differential elements between the normal leaves mixing pool(Eo)and crinkle leaves mixing pool(E1).Two materials GY_C(crinkle grade is HS)and GY_N(crinkle grade is HR)with almost the same genetic background were used to study the change of soybean leaf wrinkling in different soil layers,nutrient solution,ash,leaf pulp,after soil soaking solution,soil disinfection,nitrogen fertilizer and other treatments.And random block test design was used in these experiments.The results showed that leaves of GY_C grown in surface,middle and subsurface layers soil all could cause the GY_C leaves becoming wrinkled.GY_C leaves grown in middle layers soil were more wrinkled than other two kind layers soil.GY_C leaves treated with nutrient solution in which the nitrogen phosphorus potassium ratio was imbalanced did not become wrinkled.ICP-OES analysis results showed that the value of E0/E1 to all elements were within 1.7-fold.And the manganese element content in E,was lower than that in E0.GY_C leaves did not collapse after treatment of the substrate with leaf ash and soil soaking solution.The transplanting experiment showed that the new leaves of GY_C continued to shrink when the southern soil and substrate ratio was more than 3:1,whereas the GY_C leaf also collapsed when the southern soil and substrate ratio was less than 1:8 in the pot with re-planting,and the degree of leaf shrinkage increased as the number of re-plications increased.Dissection and observation of the root,stem,and petiole of the crumpled leaf material revealed no obvious traces of infestation.The E1 soil treated under-80,60,80,100 and 120 ℃ could not cause GY_C leaves wrinkled.In summary,microbial factors in the southern soil may be the key factors that cause the crinkling of soybean leaves in southern China.
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