Transcriptome Analysis of Regulatory Mechanism of Plant Growth Regulator S3307 on Growth and Development of Sinorhizobium shofinae
Aiming to investigate the molecular mechanism of the effect of S3307,a plant growth regulator,on the growth and development of Sinorhizobium shofinae under symbiotic conditions.Various concentrations of S3307 were compared for their effects on cell growth and microstructure of S.shofinae.The effect of S3307 treatment on the transcription level of S.shofinae nod gene was detected by qRT-PCR,which was verified by tieback experiment,and the regulatory mechanism was further clarified by transcriptome analysis.The results indicated that the addition of S3307 to the culture medium slowed down the cell growth rate,induced alterations in the microstructure and reduced the number of PHB of S.shofinae.Tieback test revealed that S3307 treatment reduced the number of nodules but increased the stem and leaf dry weight of soybean plants.Transcriptomic analysis identified 203 differentially expressed genes,with functional enrichment analysis showing that the significantly inhibited genes were mainly involved in pathways related to growth and cell structure.qRT-PCR verification showed that S3307 treatment inhibited the expression of IC52_RS29880(nodA)and IC52_RS31765(nodD)genes,which was consistent with the transcriptome sequencing results.Plant growth regulators have a certain regulatory effect on symbiotic rhizobia and soybeans.The transcriptome results showed that S3307 treatment inhibited the expression of S.shofinae growth gene,and their regulatory effects mainly occurred in cell process,localization,metabolism,stress response and growth.These research findings contribute to our assessment of the impact of plant growth regulators on soil ecological safety.
plant growth regulatorrhizobiagrowth and developmenttranscriptomics
万方数据
维普
