Bioinformatics Analysis of GmEXPB3 and GmEXPB6 and Their Expression Induced by Drought and Salt Stress in Soybean
Expansin(EXP)plays an important role in plant response to environmental stress by regulating cell wall relaxation.To explore the role of EXP genes in soybean response to drought and salt stress,two EXP genes(GmEXPB3 and GmEXPB6)in soybean were analyzed by bioinformatics,and their expression levels were analyzed by real-time fluorescent quantitative PCR(qRT-PCR).The GmEXPB3 and GmEXPB6 were located on chromosomes 3 and 11 of soybean,respectively,and encoded proteins containing 267 and 277 amino acids,respectively.The molecular weight of GmEXPB3 protein is 28.81 kDa and the theoretical isoelectric point is 5.62.The molecular weight of GmEXPB6 protein is 29.37 kDa and the theoretical isoelectric point is 4.95.GmEXPB3 and GmEXPB6 are both unstable hydrophilic proteins and localized in the cell wall.Both GmEXPB3 and GmEXPB6 proteins contained a conserved DPBB_1 domain.GmEXPB3 protein was closely related to TaEXPB10 protein in wheat,and GmEXPB6 protein was closely related to GmEXPB2 protein in soybean.Drought stress could induce the expression of GmEXPB3,but not GmEXPB6.Salt stress could induce the expression of GmEXPB3 and GmEXPB6.The promoter region of GmEXPB3 contained five stress related cis-elements.The promoter region of GmEXPB6 contained four stress related cis-elements.The results provide a theoretical basis for the study and application of stress-resistance mechanism of EXPB gene in soybean.
万方数据
维普
