Study on Key Enzyme Activities of Photosynthetic Carbon Metabolism in Soybean Varieties of Different Evolutionary Types
Photosynthetic carbon metabolism is the basic physiological metabolic pathway in soybean growth and development,which has an important impact on its own yield and quality.In order to study the photosynthetic characteristics and the activities of key enzymes of photosynthetic carbon metabolism in different evolutionary types of soybeans,we used wild,semi-wild,semi-cultivated and cultivated soybean varieties as experimental materials.The changes of photosynthetic characteristics and the evolution trend of carbon metabolism in the evolution of soybean varieties provided theoretical basis for breeding and utilization of high photosynthetic efficiency of soybeans.The results showed that the net photosynthetic rate,stomatal conductance,transpiration rate,RuBP carboxylase activity and chlorophyll content showed a single peak curve in all types of soybean varieties.The maximum net photosynthetic rate,chlorophyll content and RuBP carboxylase activity of the cultivated type was 24.2 μmol·m-2·s-1,99.9 mg·g-1 and 4.5 μmol·g-1 min-1,respectively.The activity of sucrose synthase,sucrose phosphate synthase,starch synthase,ADPG pyrophosphorylase and UDPG pyrophosphorylase were significantly higher than those of more wild,semi-wild,semi-cultivated varieties at R2 stage,which were 203.4％,67.8％,49.2％and 192.5％,142.4％,131.1％respectively.The activities of enzymes related to carbon metabolism in leaves of all types of soybean showed significant or extremely significant positive correlation with Pn at V2 and R2 stages,but no significant or negative correlation with Pn at R4,R6 and R8 stages,with the evolution of soybean varieties,the photosynthetic carbon assimilation ability of leaves was improved,and the activities of RuBP carboxylase,sucrose synthase and ADPG pyrophosphorylase were enhanced to a higher extent.The results can provide a theoretical basis for soybean breeding and high light efficiency utilization.
soybeanphotosynthetic rateRuBP carboxylasecarbon metabolism related enzymes
万方数据
维普
