Multi-omics Analysis of Carbon and Nitrogen Metabolism Pathways in Soybean Nodules under Phosphorus Stress
In order to analyze the effect of phosphorus(P)stress on nodule nitrogen fixation in soybean.In this study,soybean nodules treated with P stress for 15 days as the experimental materials,metabolomics and proteomics were conducted to analyze the differential metabolites,differential proteins,and metabolic pathways.The results indicated that:differential metabolites and proteins were up-regulated and down-regulated in response to the P stress,participating in 11 carbon metabolism pathways and 19 nitrogen metabolism pathways.Sugar substances were down-regulated and participated in carbon metabolism pathways including glycolysis,fructose and mannose metabolism,galactose metabolism,starch and sucrose metabolism,leading to insufficient energy in nodules,which affected nodule nitrogen fixation.Amino acids and enzymes were mainly up-regulated,enriched in amino acid metabolism,nitrogen metabolism,aminoacyl-tRNA biosynthesis,and purine metabolism pathways,which are the important nitrogen metabolism pathways in soybean nodules in response to P stress.The up-regulation of amino acids provided the carbon backbone for TCA cycle intermediates,and the synergistic effect of carbon and nitrogen metabolism responded to the effect of P stress on nodule nitrogen fixation in soybean.The results provide theoretical basis for analyzing the physiological mechanism of nodule nitrogen fixation under P stress.
万方数据
维普
