为了促进广谱高效大豆埃氏慢生根瘤菌Y63-1菌株的高效生产与推广应用，本研究对其培养基进行了筛选与优化。首先比较了Y63-1在YMA、TY、SM、PA和BSE 5种根瘤菌基本培养基中的生长速度，结果表明Y63-1在TY基本培养基中生长最快。以TY为基本培养基进行单因素碳源及无机盐利用试验，结果表明葡萄糖是Y63-1生长的最佳碳源，CaCl2为必要的培养基成分，Rh微量元素对菌株的生长有很大的促进作用。进一步对蛋白胨、葡萄糖、酵母粉及Rh微量元素4种组分进行正交优化，获得了适宜Y63-1生长的最佳培养基，配方(1 L)为:8 g蛋白胨、10 g葡萄糖、3 g酵母粉、0。1 g CaCl2·6H2O、3 mLRh微量元素，pH7。0。此培养基也能显著提升USDA110的生长速率，可以广泛应用于慢生根瘤菌菌剂的大规模生产。
Screening and Optimization of Culture Medium for Bradyrhizobium elkanii Y63-1
In order to promote the efficient production and application of the Y63-1 strain of soybean slow growing rhizobia with broad-spectrum and high efficiency,this study screened and optimized its culture medium.Firstly,the growth rate of Y63-1 was compared in five basic culture media for rhizobia,including YMA,TY,SM,PA and BSE.The results showed that Y63-1 grew the fastest in TY basic medium.Single factor carbon source and inorganic salt utilization experiments were conducted using TY as the basic culture medium.The results showed that glucose was the optimal carbon source for the growth of Y63-1,CaCl2 was a necessary component of the culture medium,and Rh trace elements had a significant promoting effect on the growth of the strain.Further orthogonal optimization was conducted on the four components of peptone,glucose,yeast powder,and Rh trace elements to obtain the optimal culture medium for the growth of Y63-1.The formula(1 L)was:8 g peptone,10 g glucose,3 g yeast powder,0.1 g CaCl2·6H2O,and 3 mL Rh trace elements,pH7.0.This culture medium can also significantly improve the growth rate of USDA110 and can be widely used in the large-scale production of slow growing rhizobia agents.
万方数据
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