Bioinformatics Analysis of GmHSF21 Gene in Soybean
Plant heat shock transcription factors(HSFs)played important roles in participating in stress conditions such as high temperature,drought,and salinity.To explore the role of GmHSF21 gene in salt stress response,this article took transcriptome differential gene data of soybean variety Zhonghuang 13 under pre salt stress treatment as the research object,and conducted bioinformatics analysis on candidate gene GmHSF21 in the HSF gene family.The results showed that GmHSF21 belonged to subgroup A of the HSF gene family,it encoded 341 amino acids.The coding region had four polymorphic sites,conserved HSF and HSF DNA-binding motifs.Phylogenetic analysis showed that AT3G22830 was closely related to GmHSF21.The analysis of cis-acting elements showed that the promoter region had acting elements in response to various biological and abiotic stresses.Fluorescence quantitative PCR results showed that the expression of the gene was up-regulated under salt stress,which was basically consistent with the transcriptome results.
万方数据
维普
