In order to study the effect of different EMS treatment time on the main quality content of soybean induced progenies,0.5%EMS solution was used to treat high protein soybean line Gongjiao 1057-1 for 4,7 and 10 h respectively,the changes of main quality contents in four generations of induced mutation progenies after sowing were analyzed.The results showed that compared with the control,the survival rate of EMS-treated plants decreased with the prolongation of treatment time.EMS-4 had the highest survival rate(43.5%)and EMS-10 had the lowest survival rate(16.0%).Different EMS treatment time had significant effect on protein content,but had no significant effect on fat content.The protein content of EMS-4 treatment was higher than that of the other three treatments.After EMS-4 treatment,the protein content of M2 generation was significantly separated,and the superparent individuals appeared.The change of fat content was not significant.