[Introduction] The surface quality of the inner wall of conformal cooling channel made of 316L stainless steel by selective laser melting (SLM) needs to be improved. [Method] An environmentally friendly electrolyte composed of NaCl, ethylene glycol, and triethanolamine was used to electrolytically polish the inner wall of 316L stainless steel conformal cooling channel in a cathode/anode interchanging mode. The effects of anodic current density, temperature, cathode/anode interchange frequency, and electrolysis time for each interchange on the surface roughness and microscopic morphology of the electrolytically polished specimen were studied by orthogonal test and single-factor experiment. [Result] The electrolytic polishing process parameters were optimized as follows: anodic current density 0.55 A/cm2, temperature 40 ℃, cathode/anode interchange for 3 times, and electrolysis for 10 minutes per interchange and 60 minutes in total. After being electrolytically polished under the optimized conditions, the inner wall of cooling channel was bright and smooth with a surface roughness (Sa) of 0.436 µm. [Conclusion] Electrolytic polishing can greatly improve the surface quality of conformal cooling channel prepared by SLM. The research results of this paper can provide technical reference for industrial injection mold manufacturing.