Effect of Baihe Jizi decoction on the pituitary metabolite profile of rats with depression
Objective:To investigate the changes in the metabolism of small-molecule compounds in pituitary tis-sue of a rat model of depression induced by chronic unpredicted mild stress(CUMS),as well as the metabolic pathways affected by Baihe Jizi decoction.Methods:CUMS was used to establish a model of depression.The pituitary tissue of rats was collected for magnetic resonance;the metabolomics multivariate statistical analysis was used to perform visual analysis of differentially expressed metabolites,and changes in the content of differentially expressed metabolites were used to deter-mine the metabolic pathways affected by Baihe Jizi decoction.Results:The principal component analysis showed signifi-cant differences between groups,and eight differentially expressed metabolites,including lactic acid,glutamine,and cho-line,were obtained by the multivariate statistical analysis.Compared with the normal group,the model group had significant reductions in the content of the metabolites such as lactic acid,glutamine,and choline(P＜0.05 or P＜0.01),and com-pared with the model group,the middle-dose Baihe Jizi decoction group had significant increases in the content of these metabolites(P＜0.05 or P＜0.01).Conclusion:Depression can significantly change the metabolite profile of pituitary tis-sue in rats,and Baihe Jizi decoction can exert an antidepressive effect through the pathways such as alanine,aspartate,and glutamate metabolism,D-glutamine and D-glutamic acid metabolism,and taurine and hypotaurine metabolism.
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