Antipyretic effect of Maxing Shigan decoction in a rat model of pyrexia and its effect on the hydrogen sulfide/cystathionine-β-synthase system
Objective:To investigate the antipyretic effect of Maxing Shigan decoction in a rat model of pyrexia in-duced by lipopolysaccharide(LPS)and related mechanisms,as well as its effect on the hydrogen sulfide(H2S)/cystathi-onine-β-synthase(CBS)system in rat brain tissue.Methods:A total of 50 specific pathogen-free Sprague-Dawley rats were randomly divided into blank group,control group(distilled water),aspirin group(0.125 g/kg enteric-coated aspirin tablets),low-dose Maxing Shigan decoction group(3.125 g/kg Maxing Shigan decction),and high-dose Maxing Shigan decoction group(6.250 g/kg Maxing Shigan decoction),with 10 rats in each group.The drugs were given by gavage for 5 days,and at 1 hour after the last administration by gavage,the rats in each administration group were given intraperitoneal injection of 20 μg/kg LPS solution to induce pyrexia in rats,while those in the blank group were given an equal dose of 0.9％sodium chloride injection.The change in rectal temperature was recorded within 5 hours after modeling.After meas-urement of body temperature for the last time,3％pentobarbital sodium was intraperitoneally injected for anesthesia of rats,and blood was collected via the abdominal aorta.ELISA was used to measure the serum levels of interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α),and interferon-α(IFN-α).Rat brain tissue was collected,and RT-qPCR was used to measure the mRNA expression levels of CBS and H2S in brain tissue.Results:Compared with the control group,the low-and high-dose Maxing Shigan decoction groups showed a good antipyretic effect.Both low-and high-dose Maxing Shigan decoction could inhibit the expression of IL-1 β and TNF-α in serum,and high-dose Maxing Shigan de-coction could significantly inhibit the expression of IFN-α in serum(P＜0.05).Compared with the control group,the low-and high-dose Maxing Shigan decoction groups showed significant reductions in the mRNA expression levels of CBS and H2S(P＜0.01).Conclusion:Maxing Shigan decoction has a good antipyretic effect,possibly by reducing the release of inflammatory factors in serum;meanwhile,Maxing Shigan decoction reduces the mRNA expression levels of CBS and H2S in rat brain tissue and may exert a protective effect on brain tissue.
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