In vitro antitumor activity of dulcitol on C6 glioma cells and related mechanism
Objective:To investigate the effect of dulcitol on the in vitro proliferation and apoptosis of C6 glioma cell line.Methods:Rat C6 glioma cells were randomly divided into control group,dulcitol intervention group,dulcitol+rapamycin group,and dulcitol+3-MA group,and these groups were incubated with different drugs.MTT assay was used to measure the inhibition rate of dulcitol on the in vitro proliferation of C6 cells,and the cells with a relatively high inhibition rate and good growth status were obtained;Hoechest33342 staining and transmission electron microscopy were used to observe the change in cell apoptosis and submicroscopic structure;immunofluorescence assay and Western blot were used to measure the expression levels of apoptosis-and autoph-agy-related proteins.Results:Dulcitol showed a marked inhibitory effect on the proliferation of C6 glioma cells,with a good concentration-effect relationship,and compared with the control group,the inhibition rate of cell pro-liferation increased with the increase in concentration(P＜0.01).Hoechst staining showed that compared with the control group,C6 cells incubated with dulcitol for 24 hours had marked karyopyknosis,suggesting the apopto-sis of a large number of cells.Western blot showed that compared with the control group,there was an increase in the expression of the pro-apoptotic gene Bax and a reduction in the expression of the anti-apoptotic gene Bcl-2.Conclusion:Dulcitol can inhibit the proliferation of C6 cells and induce the apoptosis of C6 cells,possibly by upregulating Bax and downregulating Bcl-2.
dulcitolC6 glioma cellsantitumor activitymechanism of action
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