Effect of Huodan Biyuan pills in inhibiting inflammatory response in rats with acute sinusitis based on the high-mobility group box 1/Toll-like receptor 4/nuclear factor-kappa B pathway
Objective:To investigate the effect of Huodan Biyuan pills on inflammation in rats with acute sinusitis and its molecular mechanism.Methods:A total of 36 Sprague-Dawley rats were divided into normal group,model group,positive drug group,and low-,middle-,high-dose Huodan Biyuan pills groups using a random number table,with 6 rats in each group.Nasal cavity occlusion combined with nasal dripping of lipopolysaccharide were performed to establish a rat model of acute sinusitis.After modeling,the rats in the positive drug group were given the dry suspension of amoxicillin and clavulanate potassium(4∶1)at a dose of 98.4375 g/kg by gavage;the rats in the low-,middle-,high-dose Huodan Biyuan pills groups were given Huodan Biyuan pills by gavage at a dose of 1.26 g/kg,2.52 g/kg,and 5.04 g/kg,respec-tively;the rats in the normal group and the model group were given 0.9％sodium chloride by gavage at a volume of 3 mL each time,twice a day for 21 consecutive days.HE staining was used to observe the inflammatory changes of sinus mucosa;ELISA was used to measure the serum levels of interleukin-1 β(IL-1 β),interleukin-6(IL-6),and tumor necrosis fac-tor-α(TNF-α),and immunohistochemistry and Western blotting were used to measure the protein expression levels of IL-1β,IL-6,TNF-α;Western blotting was used to measure the protein expression levels of high-mobility group box 1(HMGB1),Toll-like receptor 4(TLR4),nuclear factor-kappa B p65(NF-κB p65),and phosphorylated NF-κB p65(p-NF-κB p65).Results:Compared with the normal group,the model group had significant inflammatory cell infiltration in sinus mucosa,while the positive drug group and the Huodan Biyuan pills groups had a reduction in inflammatory cell in-filtration.Compared with the normal group,the model group had significant increases in the serum levels and protein ex-pression levels of IL-1 β,IL-6,and TNF-α(P＜0.05),as well as significant increases in the protein expression levels of HMGB1,TLR4,NF-κB p65,and p-NF-κB p65 in sinus mucosa(P＜0.05).Compared with the model group,the positive drug group and the Huodan Biyuan pills groups had significant reductions in the serum levels and protein expression levels of IL-1β,IL-6,and TNF-α(P＜0.05),as well as significant reductions in the protein expression levels of HMGB1,TLR4,NF-κB p65,and p-NF-κB p65 in sinus mucosa(P＜0.05).Compared with the positive drug group,the high-dose Huodan Biyuan pills group had significant reductions in the serum levels and protein expression levels of IL-1 β,IL-6,and TNF-α(P＜0.05),as well as significant reductions in the protein expression levels of HMGB1,TLR4,NF-κB p65,and p-NF-κB p65(P＜0.05).Conclusion:Huodan Biyuan pills can effectively inhibit inflammatory response in rats with acute sinusitis,possibly by downregulating HMGB1,inhibiting the TLR4/NF-κB signaling pathway,and reducing the release of IL-1 β,IL-6,and TNF-α.
acute sinusitisHuodan Biyuan pillshigh-mobility group box 1/Toll-like receptor 4/nuclear factor-kappa B
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