中药复方乳凝胶治疗痤疮的效果评价及作用机制研究
Effect and mechanism of action of compound traditional Chinese medicine emulsion gel in treatment of acne
陈伊萌 1惠丹丹 1穆阿宁 1张艳茹 1周家豪 1梁引库2
作者信息
- 1. 陕西理工大学生物科学与工程学院,陕西 汉中,723000;陕西省资源生物重点实验室,陕西 汉中,723000
- 2. 陕西理工大学生物科学与工程学院,陕西 汉中,723000;陕西省资源生物重点实验室,陕西 汉中,723000;陕南秦巴山区生物资源综合开发协同创新中心,陕西 汉中,723000
- 折叠
摘要
目的:观察中药复方乳凝胶对兔耳痤疮模型的药效作用,并初步揭示其作用机制.方法:利用油酸制备兔耳痤疮模型,确认造模成功后开始给药干预,通过病理组织切片HE染色考察复方乳凝胶的治疗效果;利用酶联免疫吸附法(ELISA)测定相关炎症因子,包括肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)和白细胞介素-8(IL-8)的表达量;利用免疫组化探究介导痤疮发病的关键信号通路[核转录因子κB(NF-κB)、白细胞分化抗原(CD36)受体、NOD样受体家族3(NLRP3)炎症小体]的表达水平.结果:HE组织染色及免疫组化结果表明复方乳凝胶治疗后兔耳痤疮病变组织得到显著改善,复方乳凝胶组血清中调控病变的炎症因子TNF-α、IL-1β、IL-6和IL-8表达量显著降低(P＜0.05或P＜0.01);且可介导痤疮发病的关键信号通路NF-κB,下调CD36受体、NLRP3炎症小体的表达水平(P＜0.05或P＜0.01).结论:中药复方乳凝胶通过抑制痤疮丙酸杆菌,使与痤疮相关的各种细胞上的TLRs受体及CD36受体表达下调,阻断了 NF-κB通路,抑制了 NLRP3炎症小体的活化表达,降低了血清中TNF-α、IL-6、IL-8及IL-1β炎症因子的表达水平,从而达到治疗痤疮的效果.
Abstract
Objective:To investigate the pharmacodynamic action of compound traditional Chinese medicine(TCM)emulsion gel on a rabbit ear acne model and its preliminary mechanism of action.Methods:Oleic acid was used to establish a rabbit ear acne model,and drug intervention was performed after successful modeling.HE stai-ning of histopathological sections was performed to analyze the therapeutic effect of compound emulsion gel;ELISA was used to measure related inflammatory factors,including tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6),and interleukin-8(IL-8);immunohistochemistry was used to measure the ex-pression levels of the key signaling pathways mediating the development of acne,including nuclear factor-kappa B(NF-κB),leukocyte differentiation antigen(CD36)receptor,and NOD-like receptor protein 3(NLRP3)inflam-masome.Results:HE staining and immunohistochemistry showed that there was a significant improvement in rabbit ear acne lesions after treatment with compound emulsion gel,and the compound emulsion gel group had significant reductions in the expression levels of the inflammatory factors TNF-α,IL-1β,IL-6,and IL-8 in serum(P＜0.05 or P＜0.01).Compound emulsion gel could mediate the key signaling pathway NF-κB in the development of acne and downregulated the expression levels of CD36 receptor and NLRP3 inflammasome(P＜0.05 or P＜0.01).Con-clusion:The compound TCM emulsion gel exerts a therapeutic effect on acne by inhibiting Propionibacterium acnes,downregulating the expression of TLRs receptor and CD36 receptor on various types of cells associated with acne,blocking the NF-κB pathway,inhibiting the activation and expression of NLRP3 inflammasome,and reducing the expression levels of the inflammatory factors TNF-α,IL-6,IL-8,and IL-1β in serum.
关键词
中药复方乳凝胶/痤疮/疗效评价/作用机制/炎症因子Key words
compound traditional Chinese medicine emulsion gel/acne/therapeutic effect/mechanism of action/inflammatory factor引用本文复制引用
基金项目
陕西理工大学重点科研机构开放课题研究基金(SLGPT2019KF04-05)
陕西省科技计划(2023-CX-PT-24)
陕西省科技计划(2023-YBSF-017)
出版年
2024
国家科技期刊平台
NSTL
万方数据