Effect of Jiangqi Pingchuan prescription on the expression of a disintegrin and metalloprotease 33 and E26 transformation-specific 1 in airway smooth muscle cells of rats with asthma
Objective:To investigate the effect of serum containing Jiangqi Pingchuan prescription on the expres-sion of the susceptibility genes a disintegrin and metalloprotease 33(ADAM33)and E26 transformation-specific 1(ETS-1)in airway smooth muscle cells(ASMCs)of rats with asthma and its mechanism of action.Methods:Ovalbumin sensitization was used to establish a rat model of asthma,and female Sprague-Dawley rats were divided into Jiangqi Ping-chuan prescription group and montelukast sodium group and were given drug-containing serum at different concentra-tions.The serum containing 5％,10％,and 15％Jiangqi Pingchuan prescription was prepared for the Jiangqi Pingchuan prescription group,and the serum containing 30％montelukast sodium was prepared for the montelukast sodium group.ASMCs cultured in vitro were assimilated and intervened using the drug-containing serums for 24 hours.CCK8 as-say was used to measure the inhibition rate of different drug-containing serums on the growth of ASMCs;ELISA was used to measure the concentration of transforming growth factor-beta 1(TGF-β1)and interleukin-6(IL-6)in the superna-tant of each group;Western blot was used to measure the protein expression levels of ADAM33 and ETS-1 in cells.Results:The cells cultured were successfully identified by cellular immunofluorescence technique.The serum contai-ning 5％and 10％Jiangqi Pingchuan prescription significantly inhibited the proliferation of ASMCs(P＜0.05 or P＜0.01).In the supernatant of ASMCs,the 10％Jiangqi Pingchuan prescription group had significant reductions in the concentration of TGF-β1 and IL-6 compared with the asthma model group(P＜0.05).Compared with the asthma model group,the 10％Jiangqi Pingchuan prescription group had significantly greater downregulation of the high expression level of ADAM33 protein(P＜0.01),and the 10％and 15％Jiangqi Pingchuan prescription groups and the 30％Western medi-cine group had significantly greater downregulation of the high expression level of ETS-1 protein(P＜0.01).Conclusion:Jiangqi Pingchuan prescription can reduce the release of the inflammatory cytokines TGF-β1 and IL-6 and improve airway remodeling by regulating the expression of ADAM33 and the transcription factor ETS-1,thereby exerting an interventional and therapeutic effect on asthma.
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