Pharmacodynamics and toxicity of a traditional Chinese medicine prescription in treatment of vaginitis:An experimental study
Objective:To investigate the pharmacodynamics and toxicity of a self-made traditional Chinese medicine(TCM)prescription in the treatment of vaginitis based on an experimental study.Methods:A total of 40 guinea pigs were randomly divided into model control group,dexamethasone positive drug group(0.25 mg/ml),high-dose TCM prescription group(52.00 mg/ml),middle-dose TCM prescription group(13.00 mg/ml),and low-dose TCM prescription group(3.25 mg/ml),with 8 guinea pigs in each group.The corresponding drug was rubbed at the instep of the right rear foot after shaving,and at 15 minutes after the last administration,different con-centrations of histamine phosphate were rubbed at the pricking site in proper order until the guinea pig turned back and licked.In the experiment with the rat model of toe swelling,the rats were divided into the same groups.The toe of the rat was injected with 3％formaldehyde to establish a model of toe swelling,and after successful modeling,the corresponding drug was rubbed at the site of the inflammation.New Zealand white rabbits were divided into exposure group and control group,with 3 rabbits in each group,and the rabbits in the exposure group were injected with 52 mg/ml TCM stock solution,while those in the control group were injected with 0.9％sodium chloride injection,once every 24 hours for 5 consecutive days.A statistical analysis was performed for the number of scratches per unit time and the total amount of histamine phosphate tolerated by the guinea pigs;regression of toe swelling was ob-served,and the change in toe thickness was recorded;vaginal edema of the experimental rabbits was observed,and HE staining was used to observe the change in vaginal mucosa.Results:Compared with the model control group,the high-,middle-,and low-dose TCM prescription groups had a significant reduction in the number of scratches per unit time and a significant increase in the total amount of histamine phosphate tolerated by the guinea pigs,and the low-dose TCM prescription group tended to have better changes,showing a significantly better effect than the dexa-methasone positive drug group(P＜0.05).The high-and middle-dose TCM prescription groups had rapid subsid-ence of swelling,with no significant difference compared with the dexamethasone positive drug group(P＞0.05).The administration group had no marked congestion or swelling of vaginal tissue and had clear layers of vaginal mucosa,with no significant differences compared with the sections of the model control group.The stimulation index of vagi-nal mucosa was 0.2 in the exposure group.Conclusion:The self-made TCM prescription has anti-inflammatory,de-tumescence,and itching-relieving effects against vaginitis,without stimulation on vaginal mucosa or obvious toxic and side effects.
vaginitistraditional Chinese medicine prescriptionpharmacodynamicstoxicity evaluation
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