Mechanism of action of Tongmaitangyanming capsules in treatment of diabetic retinopathy:A study based on network pharmacology and molecular docking
Objective:To investigate the mechanism of action of Tongmaitangyanming capsules in the treat-ment of diabetic retinopathy(DR)based on network pharmacology and molecular docking.Methods:Databases inclu-ding TCMSP,PubChem,and Swiss Target Prediction and related articles were used to obtain the main chemical components of Tongmaitangyanming capsules and their corresponding targets,and UniPort database was used to uni-fy the names of genes.Cytoscape software was used to construct a drug-compound-target network.GeneCards data-base was used to obtain the targets associated with DR,and the intersecting targets were obtained.STRING platform was used to construct a protein-protein interaction network,and Metascape platform was used to perform gene ontol-ogy functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis of the targets of active components.AutoDock Vina was used to perform molecular docking between the active compo-nents and the key targets.Results:There were 99 active components of Tongmaitangyanming capsules,which corre-sponded to 92 targets of DR.Tongmaitangyanming capsules exerted a therapeutic effect mainly through the key tar-gets such as TNF,AKT1,IL-6,VEGFA,IL-1β,TP53,and CCL2.The main pathways included the AGE-RAGE signaling pathway,the HIF-1 signaling pathway,the PI3K-Akt signaling pathway,the VEGF signaling pathway,and the NF-κB signaling pathway.The specific biological functions were reflected in the positive regulation of cytokine receptor binding,protein homodimerization activity,binding of transcription coactivators,oxidation-reduction enzyme activity,and kinase binding.Molecular docking showed good binding activity between the main active components and the target proteins.Conclusion:This study shows that Tongmaitangyanming capsules exert a therapeutic effect on DR through multiple components,targets,and pathways,which provides a theoretical basis for clinical application.
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