Regulatory mechanism of modified Shenling Baizhu powder on a guinea pig model of allergic rhinitis with dampness stagnancy due to spleen deficiency
Objective:To investigate the therapeutic effect of modified Shenling Baizhu powder on a guinea pig model of allergic rhinitis with dampness stagnancy due to spleen deficiency.Methods:A total of 30 specific pathogen-free guinea pigs were selected,and the method of ovalbumin sensitization was used to establish a model of allergic rhinitis,while alternate intragastric administration of zero-degree water and animal oil combined with stand-ing in water were performed to establish a model of dampness stagnancy due to spleen deficiency.After successful modeling,the guinea pigs were randomly divided into model group(0.9％sodium chloride injection,4 mL per guinea pig),Western medicine treatment group(loratadine,1 mL/0.1 kg),and traditional Chinese medicine treat-ment group(modified Shenling Baizhu powder,1 mL/0.1 kg),with 10 guinea pigs in each group.Another 10 guinea pigs were selected as blank control group(0.9％sodium chloride injection,4 mL per guinea pig).After the corresponding intervention,behavioral observation,HE staining,and ELISA were used to assess the symptoms of guinea pigs in each group,the histomorphology of nasal mucosa,and the levels of nasal mucosa-related factors[Eotaxin,myelin basic protein(MBP),and interleukin-4(IL-4)]and serum factors[IL-4,interleukin-5(IL-5),and interleukin-17(IL-17)].Results:Compared with the blank control group,the model group showed marked symptoms and histological injury of allergic rhinitis,as well as significant increases in the levels of Eotaxin,MBP,and IL-4 in nasal mucosa and the serum levels of IL-4,IL-5,and IL-17(P＜0.05).Compared with the model group,the Western medicine treatment group and the traditional Chinese medicine treatment group had significant improvements in behavioral score and histomorphology and significant reductions in the levels of Eotaxin,MBP,and IL-4 in nasal mucosa and the serum levels of IL-4,IL-5,and IL-17(P＜0.05).Conclusion:Modified Shenling Baizhu powder can significantly improve the disordered structure of nasal mucosa and alleviate inflammatory re-sponse in nasal mucosa in guinea pigs with dampness stagnancy due to spleen deficiency,suggesting that modified Shenling Baizhu powder has a marked therapeutic effect on allergic rhinitis,but further studies are needed to clarify its exact mechanism.
allergic rhinitisdampness stagnancy due to spleen deficiencymodified Shenling Baizhu powder
万方数据
维普
