摘要
目的:观察针刺法对抑郁症模型大鼠行为学的影响.方法:随机选取24只雄性大鼠制备孤养结合慢性不可预见性温和刺激抑郁症模型,造模成功后将其分为模型组、氟西汀组、针刺组,每组各8只.随机选取正常雄性大鼠8只作为空白对照组.空白对照组、模型组每日上午9:00予0.9％氯化钠注射液灌胃;在此基础上,针刺组予百会穴、关元穴针刺干预;氟西汀组每日上午9:00予以氟西汀药液1.8 mg/kg灌胃.分别在干预的第1、7、14、21、28天对各组大鼠进行Morris水迷宫测试,记录大鼠自我修饰、区域探视、跟随同伴、身体接触的次数,观察大鼠行为学及学习记忆的情况.结果:干预后,模型组、氟西汀组、针刺组大鼠潜伏期明显高于空白对照组(P＜0.05),且干预第21天,氟西汀组、针刺组潜伏期低于模型组(P＜0.05或P＜0.01).模型组、氟西汀组、针刺组大鼠干预第7、14天水迷宫穿过平台次数少于空白对照组(P＜0.05),且针刺组与模型组第1、14、21、28天比较,差异有统计学意义(P＜0.05或P＜0.01).模型组、氟西汀组、针刺组第1、21、28天跟随同伴次数、自我修饰次数、同伴身体接触次数、区域探视次数均少于空白对照组(P＜0.05),上述指标,氟西汀组干预第14天与模型组同时间节点比较,差异有统计学意义(P＜0.05或P＜0.01).结论:针刺法对抑郁症大鼠的行为学以及学习记忆有一定的调节和改善作用.
Abstract
Objective:To investigate the effect of acupuncture on behavioristics in a rat model of depres-sion.Methods:A total of 24 male rats were randomly selected to establish a model of depression by social isolation combined with chronic unpredictable mild stress,and after successful modeling,they were divided into model group,fluoxetine group,and acupuncture group,with 8 rats in each group.Another 8 normal male rats were randomly se-lected as blank control group.The rats in the blank control group and the model group were given 0.9％sodium chloride injection by gavage at 9:00 every morning;in addition,the rats in the acupuncture group were given acu-puncture at Baihui and Guanyuan points,and those in the fluoxetine group were given fluoxetine drug solution 1.8 mg/kg by gavage at 9:00 every morning.On days 1,7,14,21,and 28 of intervention,the Morris water maze test was performed for the rats in each group to record the number of times of self-modification,region visit,follow-ing a partner,and body contact,and the rats were observed in terms of behavioristics and learning and memory abili-ties.Results:After intervention,the model group,the fluoxetine group,and the acupuncture group had a significantly shorter latency than the blank control group(P＜0.05),and on day 21 of intervention,the fluoxetine group and the acupuncture group had a significantly shorter latency than the model group(P＜0.05 or P＜0.01).On days 7 and 14 of intervention,the model group,the fluoxetine group,and the acupuncture group had a significantly lower num-ber of platform crossings in the water maze test than the blank control group(P＜0.05),and there was a significant difference between the acupuncture group and the model group on days 1,14,21,and 28 of intervention(P＜0.05 or P＜0.01).Compared with the blank control group,the model group,the fluoxetine group,and the acupuncture group had significantly lower numbers of times of following a partner,self-modification,partner body contact,and region visit(P＜0.05),and there were significant differences in these indicators between the fluoxetine group and the model group on day 14 of intervention(P＜0.05 or P＜0.01).Conclusion:Acupuncture can regulate and im-prove behavioristics and learning and memory abilities in rats with depression.
基金项目
湖南省卫生健康委员会科研计划项目(D202319018555)
湖南省大学生创新创业训练计划项目(S202210541107)
湖南中医药大学校级科研基金项目(2019XJJJ011)
维普
万方数据