Establishment of the high-performance liquid chromatography fingerprint of Tetradium ruticarpum and quality assessment
Objective:To establish the high-performance liquid chromatography(HPLC)fingerprint of Tetradium ruticarpum,and to provide a basis for its quality assessment.Methods:A total of 22 batches of Tetradium ruticarpum were collected from three provinces,and HPLC was performed to establish the fingerprints.The chroma-tographic fingerprint similarity evaluation system(2012 edition)and IBM SPSS Statistics 26 software were used to perform similarity evaluation,cluster analysis(CA),and principal component analysis(PCA),and the 1,1-diphenyl-2-picrylhydrazyl hydrate method was used to measure the antioxidant activity of Tetradium ruticarpum.Results:The HPLC fingerprints were established for the 22 batches of samples,with a similarity of 0.603～0.992,and a total of 13 common peaks were determined,among which 6 peaks were identified.With evodiamine as the ref-erence peak,the relative peak area was calculated for each common peak,with a relative standard deviation(RSD)of 45.29％～112.17％,suggesting that there were great fluctuations in the content of some common components across different batches of samples.The results of similarity analysis showed the highest degree of similarity in the medicinal material from Guizhou producing areas,followed by that from Hunan producing areas,while the lowest de-gree of similarity was observed in the medicinal material from Jiangxi producing areas.The results of CA showed that the 22 batches of samples were clustered into 2 categories,with 3 batches from Hunan,6 batches from Jiangxi,and 3 batches from Guizhou as the first category and the remaining 9 batches from Jiangxi and the remaining 1 batch from Guizhou as the second category.The results of PC A showed that the top three principal components had a cumula-tive variance contribution rate of 78.164％and could be used as assessment indices for the overall quality of Tetra-dium ruticarpum.There was a difference in the antioxidant activity of Tetradium ruticarpum across different batches;the batches from Hunan producing areas showed the least difference in free radical clearance rate(RSD=9.32％),while those from Guizhou producing areas showed the greatest difference(RSD=70.17％),and those from Jiangxi producing areas showed a moderate difference(RSD=41.94％).Conclusion:The HPLC fingerprint analysis of Tet-radium ruticarpum is simple and rapid and can be used for the quality assessment of medicinal material.Tetradium ruticarpum from Guizhou producing areas has a relatively high degree of similarity,that from Jiangxi producing areas has the best quality,and that from Hunan producing areas has the most stable quality.Due to the difference in sam-ple size,more research data are needed to achieve a final conclusion.
万方数据
维普
